Nature Communications (Jan 2019)

The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase

  • Thomas Wiegand,
  • Riccardo Cadalbert,
  • Denis Lacabanne,
  • Joanna Timmins,
  • Laurent Terradot,
  • Anja Böckmann,
  • Beat H. Meier

DOI
https://doi.org/10.1038/s41467-018-07968-3
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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DnaB helicases are motor proteins that couple ATP-hydrolysis to the movement of the protein along single-stranded DNA leading to a separation of double-stranded DNA at the replication fork. Here authors use solid-state NMR spectroscopy and reveal DnaB’s conformational responses to ATP hydrolysis and the resulting DNA loading and translocation.