Nature Communications (Jan 2019)
The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase
Abstract
DnaB helicases are motor proteins that couple ATP-hydrolysis to the movement of the protein along single-stranded DNA leading to a separation of double-stranded DNA at the replication fork. Here authors use solid-state NMR spectroscopy and reveal DnaB’s conformational responses to ATP hydrolysis and the resulting DNA loading and translocation.