CONCENTRATIONS OF PROGESTERONE AND ESTRADIOL-17Β IN THE FOLLICULAR FLUID OF OVARIAN FOLLICLES OF GOAT

Abstract

The aim of the present study was to quantify the Progesterone (P4 ) and Estradiol17β (E2 ) in follicular fluid (FF) of medium (Gp-I; 3-5mm diameter) and large (Gp-II; more than 5mm diameter) goat ovarian follicles, by enzymelinked immune sorbent assay (ELISA). Pooled samples (n=7) of FF from two groups of follicles were used for the study. The E2 concentrations (ng/mL) in FF of Gp-I and Gp-II follices were 12.08±2.78 and 22.08±1.97 respectively. The P4 concentrations (ng/mL) in FF of Gp-I and Gp-II follicles were 10.56±2.3 and 20.53±3.82 respectively. The E2 and P4 concentrations were significantly (p≤0.05) higher in the Gp-II follicles than the Gp-I follicles. Similarly, increase in P4 concentration in Gp-II follicles might be due to the production of P4 in the synthetic pathway of follicular production of E2

Keywords

• progesterone
• estradiol-17β
• follicular fluid