Collateral activity of the CRISPR/RfxCas13d system in human cells

Affiliations

Peiguo Shi: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Michael R. Murphy: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Alexis O. Aparicio: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Jordan S. Kesner: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Zhou Fang: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Ziheng Chen: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Aditi Trehan: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Yang Guo: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center
Xuebing Wu: Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center

Abstract

The collateral activity of the CRISPR/RfxCas13d system in mammalian cells is explored using spike-in RNA-seq and reporter assays.