Minimum Alveolar Concentration of Isoflurane in Rats Chronically Treated with the Synthetic Cannabinoid WIN 55,212-2
Julio Raul Chavez-Monteagudo,
José Antonio Ibancovichi,
Pedro Sanchez-Aparicio,
Sergio Recillas-Morales,
Jorge Osorio-Avalos,
Marco Antonio De Paz-Campos
Affiliations
Julio Raul Chavez-Monteagudo
Department of Veterinary Anesthesia and Analgesia, Facultad de Estudios Superiores Cuautitlán, Hospital de Pequeñas Especies, Universidad Nacional Autónoma de México, Cuautitlan Izcalli 54740, Mexico
José Antonio Ibancovichi
Department of Veterinary Anesthesia and Analgesia, Faculty of Veterinary Medicine, Universidad Autónoma del Estado de México, Toluca 50000, Mexico
Pedro Sanchez-Aparicio
Department of Pharmacology, Faculty of Veterinary Medicine, Universidad Autónoma del Estado de México, Toluca 50000, Mexico
Sergio Recillas-Morales
Department of Pharmacology, Faculty of Veterinary Medicine, Universidad Autónoma del Estado de México, Toluca 50000, Mexico
Jorge Osorio-Avalos
Department of Biostatistics, Faculty of Veterinary Medicine, Universidad Autónoma del Estado de México, Toluca 50000, Mexico
Marco Antonio De Paz-Campos
Department of Pharmacology, Facultad de Estudios Superiores Cuautitlán, Hospital de Pequeñas Especies, Universidad Nacional Autónoma de México, Cuautitlan Izcalli 54740, Mexico
The minimum alveolar concentration MAC of isoflurane was measured in rats chronically treated with WIN 55,212-2. Methods: The MAC of isoflurane was determined in 24 male rats from expiratory samples at time of tail clamping under the following conditions: without treatment MAC(ISO), in rats treated for 21 days with WIN 55,212-2 MAC(ISO + WIN55), and in rats 8 days after stopping treatment with WIN 55,212-2 (MACISO + WIN55 + 8D). Results: The MAC(ISO) was 1.32 ± 0.06. In the MAC(ISO + WIN55) group, the MAC increased to 1.69 ± 0.09 (28%, p-value ≤ (0.0001). Eight days after stopping treatment with WIN55, the MAC did not decrease significantly, 1.67 ± 0.07 (26%, p-value ≤ 0.0001). Conclusions: The administration of WIN 55,212-2 for 21 days increases the MAC of isoflurane in rats. This effect does not disappear 8 days after discontinuation of treatment with the synthetic cannabinoid.
