International Journal of General Medicine (Nov 2022)

Prognostic and Immunological Roles of Cell Cycle Regulator CDCA5 in Human Solid Tumors

  • He J,
  • Zhou X,
  • Wang X,
  • Zhang Q,
  • Zhang L,
  • Wang T,
  • Zhu W,
  • Liu P,
  • Zhu M

Journal volume & issue
Vol. Volume 15
pp. 8257 – 8274

Abstract

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Jing He,1,* Xin Zhou,1,* Xiaping Wang,2,* Qing Zhang,3 Lan Zhang,4,5 Tongshan Wang,1 Wei Zhu,1 Ping Liu,1 Mingxia Zhu6 1Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People’s Republic of China; 2Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, People’s Republic of China; 3Department of Neurosurgery, Xinghua People’s Hospital, Xinghua, Jiangsu, 225700, People’s Republic of China; 4Department of Radiotherapy & Oncology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, People’s Republic of China; 5Department of Radiation Oncology, Shanghai Tenth People’s Hospital of Tongji University, Shanghai, People’s Republic of China; 6Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mingxia Zhu; Ping Liu, Email [email protected]; [email protected]: There have been several studies evaluating the prognostic significance of cell division cycle associated 5 (CDCA5). However, few reports analyzed the correlation between CDCA5 and prognosis of diverse cancers based on large clinical data. We thus comprehensively analyzed CDCA5 expression and clinical significance using The Cancer Genome Atlas (TCGA) data from 31 types of solid tumors.Methods: The expression profiles of CDCA5 were investigated across pan-cancer samples from the TCGA. Cox regression and Kaplan–Meier analysis was performed to determine CDCA5’s prognostic value. CDCA5 expression was further validated by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) in lung adenocarcinoma (LUAD).Results: We found that CDCA5 was significantly overexpressed in 22 types of tumors. Up-regulated CDCA5 was significantly related to poor survival in 13 types of tumors. Furthermore, CDCA5 expression was significantly associated with immune cell infiltration. Tumor mutation burden (TMB), microsatellite instability (MSI), and immune checkpoint expression were significantly correlated with CDCA5 expression. Additional analysis of IMvigor 210 cohort validated that patients with high level of CDCA5 had superior response to anti-PD-L1 therapy.Conclusion: Our findings suggested that CDCA5 could provide prognostic information in most types of cancers and contributed to tumor immune microenvironment.Keywords: cell division cycle associated 5, pan-cancer, prognosis, immune infiltration, tumor mutation burden, microsatellite instability

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