Genotype × ammonia interaction effects on plasma physiological indexes in turbot (Scophthalmus maximus) cultured under acute ammonia stress for different periods of times

Abstract

We evaluated six plasma physiological indexes (epinephrine, cortisol, alkaline phosphatase, superoxide dismutase, reduced glutathione, and blood glucose) in turbot (Scophthalmus maximus) cultured in five ammonia concentrations (30.28, 35.90, 42.56, 50.47, and 59.82) at different exposure times (4, 8, 12, 24, and 48 h). Then, we conducted additive main effects and multiplicative interaction (AMMI) and genotype main effects and genotype × environment interaction (GGE) biplot analyses to analyze the genotype × ammonia interaction effects on the plasma physiological indexes. The AMMI analysis results revealed that the contributions of ammonia effect first decreased sharply (4–8 h), then decreased slowly (8–24 h), and then increased slowly (24–48 h). The contributions of genotype effect first decreased sharply (4–8 h), then increased slowly (8–24 h), and then decreased slowly (24–48 h). The contributions of genotype × ammonia interactions showed a sharp rise (4–8 h), a sharp decline (8–12 h), a slow decline (12–24 h), and finally a slow increase (24–48 h). The GGE biplot analysis revealed that alkaline phosphatase and reduced glutathione contents/activities are reliable biomarkers that can be used to establish an online early warning system for behavior numerical simulation.

Keywords

• Turbot
• Acute ammonia stress
• Plasma physiological indexes
• Ammonia concentration
• Interactions