Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products

Zuwei Xu; Lihao Ji; Wenxiu Tang; Liang Guo; Cong Gao; Xiulai Chen; Jia Liu; Guipeng Hu; Liming Liu

Engineering Microbiology (Jun 2022)

Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products

Zuwei Xu,
Lihao Ji,
Wenxiu Tang,
Liang Guo,
Cong Gao,
Xiulai Chen,
Jia Liu,
Guipeng Hu,
Liming Liu

Affiliations

Zuwei Xu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Lihao Ji: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Wenxiu Tang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Liang Guo: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Cong Gao: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Xiulai Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Jia Liu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Guipeng Hu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China
Liming Liu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Corresponding author.

Journal volume & issue: Vol. 2, no. 2
p. 100022

Abstract

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The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic gene clusters (BGCs), and poorly characterized metabolic mechanisms limit efficient production of natural products. Therefore, a metabolic engineering strategy, including traditional and emerging tools from different disciplines, was developed to further enhance natural product synthesis by Streptomyces. Here, current trends in systems metabolic engineering, including tools and strategies, are reviewed. Particularly, this review focuses on recent developments in the selection of methods for regulating the Streptomyces life cycle, strategies for the activation of silent gene clusters, and the exploration of regulatory mechanisms governing antibiotic production. Finally, future challenges and prospects are discussed.

Published in Engineering Microbiology

ISSN: 2667-3703 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: https://www.sciencedirect.com/journal/engineering-microbiology

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