circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression

Wen Su; Yuehong Shen; Yufan Wang; Feng Wang; Xia Hong; Yuling Chen; Yuntao Lin; Hongyu Yang

Molecular Therapy: Nucleic Acids (Mar 2021)

circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression

Wen Su,
Yuehong Shen,
Yufan Wang,
Feng Wang,
Xia Hong,
Yuling Chen,
Yuntao Lin,
Hongyu Yang

Affiliations

Wen Su: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China; State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 430079 Wuhan, China
Yuehong Shen: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China; Corresponding author: Yuehong Shen, Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
Yufan Wang: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China
Feng Wang: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China
Xia Hong: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China
Yuling Chen: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China
Yuntao Lin: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China
Hongyu Yang: Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China; Guangdong Provincial High-Level Clinical Key Specialty, Guangzhou, China; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Guangzhou, China; Corresponding author: Hongyu Yang, Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Journal volume & issue: Vol. 23
pp. 185 – 199

Abstract

Read online

Circular RNA (circRNA) is a newly discovered class of noncoding RNAs that plays key regulatory role in pathological development, including the regulation of several solid tumors. However, the effects of circRNA expression on oral squamous cell carcinoma (OSCC) remain unclear. With the use of high-throughput RNA sequencing data on eight paired oral cancer and adjacent healthy tissues, we observed that circRNA derived from the gene encoding pleckstrin homology domain-interacting protein (circPHIP) was highly expressed in OSCC. Additionally, circPHIP was highly expressed in other OSCC-related cell lines and was associated with tumor metastasis, TNM stage, and human papilloma virus infection status. The inhibition of circPHIP expression reduced OSCC cell migration, invasion, and proliferation. We found that circPHIP could adsorb microRNA (miR)-142-5p and upregulate the expression of PHIP and alpha-actinin 4 (ACTN4), both of which are potential oncogenes closely related to OSCC prognosis. The inhibition of miR-142-5p or overexpressing PHIP or ACTN4 reversed the circPHIP depletion-induced attenuation of OSCC malignancy. In conclusion, circPHIP is overexpressed in OSCC and enhances its malignancy via an miR-142-5p/PHIP-ACTN4/AKT serine/threonine kinase 1 signaling axis. Therefore, circPHIP may represent a novel target for treating OSCC.

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

About the journal

Abstract

Keywords