An extended U2AF65–RNA-binding domain recognizes the 3′ splice site signal

Anant A. Agrawal; Enea Salsi; Rakesh Chatrikhi; Steven Henderson; Jermaine L. Jenkins; Michael R. Green; Dmitri N. Ermolenko; Clara L. Kielkopf

doi:10.1038/ncomms10950

Nature Communications (Mar 2016)

An extended U2AF65–RNA-binding domain recognizes the 3′ splice site signal

Anant A. Agrawal,
Enea Salsi,
Rakesh Chatrikhi,
Steven Henderson,
Jermaine L. Jenkins,
Michael R. Green,
Dmitri N. Ermolenko,
Clara L. Kielkopf

Affiliations

Anant A. Agrawal: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Enea Salsi: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Rakesh Chatrikhi: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Steven Henderson: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Jermaine L. Jenkins: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Michael R. Green: Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School
Dmitri N. Ermolenko: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry
Clara L. Kielkopf: Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry

DOI: https://doi.org/10.1038/ncomms10950
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 14

Abstract

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The pre-mRNA splicing factor U2AF65 recognizes 3′ splice sites in human gene transcripts, but the details are not fully understood. Here, the authors report U2AF65 structures and single molecule FRET that reveal mechanistic insights into splice site recognition.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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