Influence of Yeast Bioinoculant Isolated from Indian Date Palm Tree (Phoenix sylvestris) Sap on the Health of Wheat Crop and Soil

Abstract

In this study, three promising yeast isolates were isolated from the sap of the Indian date palm tree (Phoenix sylvestris) and characterized by biochemical tests and 18S rRNA gene sequencing. They were confirmed as Saccharomyces cerevisiae and were designated as strains PYS-1, PYS-2, and PYS-3. These confirmed strains were used for the preparation of bioinoculants. Bioinoculant was prepared and applied to wheat crops, and the effect of Bioinoculant. Statistical analysis is carried out using analysis of variance (ANOVA), and it is found that the absorbance of chlorophyll, protein, and Indole Acetic Acid (IAA) content is significantly increased. The treatment of bioinoculant showed that crops significantly increased chlorophyll, protein, and IAA content. Further, we applied bioinoculant on the soil and measured the soil’s humus content before and after the treatment of bioinoculant. Then, a paired t-test was applied to check the effectiveness of the treatment, and it was found to significantly increase humus content in the soil. The use of bioinoculants is an economically feasible and eco-friendly method.

Keywords

• phoenix sylvestris, saccharomyces cerevisiae, 18s rrna gene sequencing, bioinoculant