Modified method for obtaining a concentrate with a high total content of phycocyanins from A. platensis

Abstract

The work proposes a modified method for obtaining phycocyanin concentrate including extraction of biomass of the food cyanobacterium Arthrospira platensis and subsequent stages of membrane processing, involving combined use of double microfiltration and single- stage ultrafiltration. Extraction of A. platensis biomass was carried out at a temperature of +40°C for 3 hours in potassium phosphate buffer, and the purity of the extract was 0.61. Double microfiltration of the extract and ultrafiltration increased the purity of the intermediate product to 2.7. The content of C-phycocyanin and allophycocyanin in the dry concentrate was (63.6±1.0)% and (4.3±0.4)%, respectively. The molecular weight distribution of protein and peptide fractions of intermediate and final products was characterized using high-pressure size exclusion liquid chromatography.