Neurolocomotor Behavior and Oxidative Stress Markers of Thiazole and Thiazolidinedione Derivatives against <i>Nauphoeta cinerea</i>
Pedro Silvino Pereira,
Adrielle Rodrigues Costa,
Thalyta Julyanne Silva de Oliveira,
Carlos Vinícius Barros Oliveira,
Maria do Carmo Alves de Lima,
Jamerson Ferreira de Oliveira,
Bonglee Kim,
Henrique D. M. Coutinho,
Antonia Eliene Duarte,
Jean Paul Kamdem,
Teresinha Gonçalves da Silva
Affiliations
Pedro Silvino Pereira
Department of Antibiotics, Federal University of Pernambuco (UFPE), Prof. Artur de Sa Avenue, University City, Recife 54740-520, PE, Brazil
Adrielle Rodrigues Costa
Department of Biological Sciences, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Thalyta Julyanne Silva de Oliveira
Department of Biological Sciences, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Carlos Vinícius Barros Oliveira
Department of Biological Sciences, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Maria do Carmo Alves de Lima
Department of Antibiotics, Federal University of Pernambuco (UFPE), Prof. Artur de Sa Avenue, University City, Recife 54740-520, PE, Brazil
Jamerson Ferreira de Oliveira
Institute of Health Sciences, Auroras Campus, University of International Integration of Afro-Brazilian Lusophony (UNILAB), 3 Abolition Avenue, Downtown, Redenção 62790-000, CE, Brazil
Bonglee Kim
Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea
Henrique D. M. Coutinho
Department of Biological Chemistry, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Antonia Eliene Duarte
Department of Biological Sciences, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Jean Paul Kamdem
Department of Biological Sciences, Regional University of Cariri (URCA), 1161 Cel. Antonio Luiz Avenue, Pimenta, Crato 63105-000, CE, Brazil
Teresinha Gonçalves da Silva
Department of Antibiotics, Federal University of Pernambuco (UFPE), Prof. Artur de Sa Avenue, University City, Recife 54740-520, PE, Brazil
Thiazolidine compounds NJ20 {(E)-2-(2-(5-bromo-2-methoxybenzylidene)hydrazinyl)-4-(4-nitrophenyl)thiazole} and NW05 [(2-(benzo (d) (1,3) dioxol-4-ylmethylene)-N-(4-bromophenyl)-thiosemicarbazone] potentiated the effect of norfloxacin in resistant bacteria; however, there are no reports on their effects on Nauphoeta cinerea in the literature. The objective of this work was to evaluate the behavioral effects and oxidative markers of NW05 and NJ20 in lobster cockroach N. cinerea. To evaluate the behavioral study, a video tracking software was used to evaluate the locomotor points and the exploratory profile of cockroaches in the horizontal and vertical regions of a new environment. The total concentration of thiol and reduced glutathione (GSH), substances reactive to thiobarbituric acid (TBARS), free iron (II) content and mitochondrial viability were determined. The antioxidant potential was evaluated by the DPPH method. Both substances induced changes in the behavior of cockroaches, showing a significant reduction in the total distance covered and in the speed. In the cell viability test (MTT), there was a significant reduction for NJ20 (1 mM). NJ20 caused a significant increase in total levels of thiol and non-protein thiol (NPSH), although it also slightly increased the content of malondialdehyde (MDA). Both compounds (NW05 and NJ20) caused a significant reduction in the content of free iron at a concentration of 10 mM. In conclusion, the compound NJ20 caused moderate neurotoxicity (1 mM), but had good antioxidant action, while NW05 did not show toxicity or significant antioxidant activity in the model organism tested. It is desirable to carry out complementary tests related to the antioxidant prospection of these same compounds, evaluating them at different concentrations.
