Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from <em>Eupatorium lindleyanum</em> DC. by High-Speed Counter-Current Chromatography

Abstract

A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from <em>Eupatorium lindleyanum</em> DC. The two-phase solvent system composed of <em>n</em>-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the <em>n</em>-butanol fraction of <em>Eupatorium lindleyanum </em>DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and ¹H-NMR.

Keywords

• &lt;em&gt;Eupatorium lindleyanum&lt;/em&gt; DC.
• high-speed counter-current chromatography
• sesquiterpenoid lactone
• separation