Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in Mortierella alpina

Chengfeng Ge; Chengfeng Ge; Haiqin Chen; Haiqin Chen; Tiantian Mei; Tiantian Mei; Xin Tang; Xin Tang; Lulu Chang; Lulu Chang; Zhennan Gu; Zhennan Gu; Hao Zhang; Hao Zhang; Wei Chen; Wei Chen; Wei Chen; Wei Chen; Yong Q. Chen; Yong Q. Chen; Yong Q. Chen; Yong Q. Chen

doi:10.3389/fbioe.2017.00089

Frontiers in Bioengineering and Biotechnology (Jan 2018)

Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in Mortierella alpina

Chengfeng Ge,
Chengfeng Ge,
Haiqin Chen,
Haiqin Chen,
Tiantian Mei,
Tiantian Mei,
Xin Tang,
Xin Tang,
Lulu Chang,
Lulu Chang,
Zhennan Gu,
Zhennan Gu,
Hao Zhang,
Hao Zhang,
Wei Chen,
Wei Chen,
Wei Chen,
Wei Chen,
Yong Q. Chen,
Yong Q. Chen,
Yong Q. Chen,
Yong Q. Chen

Affiliations

Chengfeng Ge: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Chengfeng Ge: School of Food Science and Technology, Jiangnan University, Wuxi, China
Haiqin Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Haiqin Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Tiantian Mei: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Tiantian Mei: School of Food Science and Technology, Jiangnan University, Wuxi, China
Xin Tang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Xin Tang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Lulu Chang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Lulu Chang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Zhennan Gu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Zhennan Gu: School of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China
Wei Chen: Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, China
Yong Q. Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Yong Q. Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Yong Q. Chen: Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, China
Yong Q. Chen: Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, United States

DOI: https://doi.org/10.3389/fbioe.2017.00089
Journal volume & issue: Vol. 5

Abstract

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In the industrial oleaginous fungus Mortierella alpina, the arachidonic acid (AA; C20:4; ω-6) fraction can reach 50% of the total fatty acids (TFAs) in vivo. However, the eicosapentaenoic acid (EPA; C20:5; ω-3) fraction is less than 3% when this fungus is cultivated at a low temperature (12°C). Omega-3 fatty acid desaturase is a key enzyme in ω-3 long-chain polyunsaturated fatty acids biosynthesis pathways. To enhance EPA production, we transformed the ω-3 fatty acid desaturase (PaD17), which exhibits strong Δ-17 desaturase activity, into M. alpina, thus increasing the AA to EPA conversion rate to 49.8%. This PaD17-harboring M. alpina reconstruction strain produced 617 mg L−1 of EPA at room temperature in broth medium, this yield was increased to 1.73 g L−1 after culture medium optimization (i.e., about threefold higher than that under original culture conditions), with concomitant respective increases in dry cell weight and TFA content to 16.55 and 6.46 g L−1. These findings suggest a new platform for the future industrial production of EPA.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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