Cancer Management and Research (Mar 2021)
MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712
Abstract
Yifan Yu, Wenshan Zuo, Wei Cai, Yong Xu, Weidong Liu, Zexue Zhao Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an City, Nanjing Province, 223300, People’s Republic of ChinaCorrespondence: Wenshan ZuoDepartment of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Nanjing Province, 223300, People’s Republic of ChinaEmail [email protected]: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.Results: Lnc712 was upregulated in OS and was inversely correlated with miR-129-5p. In OS cells, Lnc712 overexpression failed to significantly affect miR-129-5p, while miR-129-5p overexpression led to downregulated Lnc712. Cell proliferation showed that Lnc712 overexpression resulted in increased cell proliferation rate. MiR-129-5p overexpression played an opposite role and reversed the effect of Lnc712 overexpression.Discussion: MiR-129-5p may suppress cell proliferation of OS by down-regulating Lnc712.Keywords: Lnc712, osteosarcoma, miR-129-5p, proliferation
