Nature Communications (Apr 2018)

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling

  • Shi-Rong Hong,
  • Cuei-Ling Wang,
  • Yao-Shen Huang,
  • Yu-Chen Chang,
  • Ya-Chu Chang,
  • Ganesh V. Pusapati,
  • Chun-Yu Lin,
  • Ning Hsu,
  • Hsiao-Chi Cheng,
  • Yueh-Chen Chiang,
  • Wei-En Huang,
  • Nathan C. Shaner,
  • Rajat Rohatgi,
  • Takanari Inoue,
  • Yu-Chun Lin

DOI
https://doi.org/10.1038/s41467-018-03952-z
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells, therefore assessing the physiological roles in specific subcellular compartments has been challenging. Here the authors develop a method to rapidly deplete tubulin glutamylation inside the primary cilia by targeting an engineered deglutamylase to the axoneme.