Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
Jeremy John Mathan,
Salim Ismail,
Jennifer Jane McGhee,
Trevor Sherwin
Affiliations
Jeremy John Mathan
Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health Sciences, the University of Auckland, Private Bag 92019, Auckland, New Zealand
Salim Ismail
Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health Sciences, the University of Auckland, Private Bag 92019, Auckland, New Zealand
Jennifer Jane McGhee
Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health Sciences, the University of Auckland, Private Bag 92019, Auckland, New Zealand
Trevor Sherwin
Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health Sciences, the University of Auckland, Private Bag 92019, Auckland, New Zealand
Stem and progenitor cells isolated from human limbal tissue can be cultured in vitro as spheres. These spheres have potential for use as transplantable elements for the repopulation of corneal tissue (Mathan et al., 2016). Herein we describe the detailed protocol for the implantation of human corneal spheres into cadaveric human corneal tissue. This protocol describes the procedure for sphere formation and culture, preparation of tissue for sphere implantation, corneal limbus microsurgery and sphere implantation.
