Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions
Manuela Kramer,
Melvin Rodriguez-Heredia,
Francesco Saccon,
Laura Mosebach,
Manuel Twachtmann,
Anja Krieger-Liszkay,
Chris Duffy,
Robert J Knell,
Giovanni Finazzi,
Guy Thomas Hanke
Affiliations
Manuela Kramer
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom; Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück, Osnabrück, Germany
Melvin Rodriguez-Heredia
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom
Francesco Saccon
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom
Laura Mosebach
Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany
Manuel Twachtmann
Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück, Osnabrück, Germany
Anja Krieger-Liszkay
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, Paris, France
Chris Duffy
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom
Giovanni Finazzi
Laboratoire de Physiologie Cellulaire et Végétale, UMR 5168, Centre National de la Recherche Scientifique (CNRS), Commissariat a` l’Energie Atomique et aux Energies Alternatives (CEA), Université Grenoble Alpes, Institut National Recherche Agronomique (INRA), Institut de Recherche en Sciences et Technologies pour le Vivant (iRTSV), CEA Grenoble, Grenoble, France
School of Biochemistry and Chemistry, Queen Mary University of London, London, United Kingdom; Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück, Osnabrück, Germany
During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.
