Nature Communications (Jan 2020)
Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection
- Bo Li,
- Sara M. Clohisey,
- Bing Shao Chia,
- Bo Wang,
- Ang Cui,
- Thomas Eisenhaure,
- Lawrence D. Schweitzer,
- Paul Hoover,
- Nicholas J. Parkinson,
- Aharon Nachshon,
- Nikki Smith,
- Tim Regan,
- David Farr,
- Michael U. Gutmann,
- Syed Irfan Bukhari,
- Andrew Law,
- Maya Sangesland,
- Irit Gat-Viks,
- Paul Digard,
- Shobha Vasudevan,
- Daniel Lingwood,
- David H. Dockrell,
- John G. Doench,
- J. Kenneth Baillie,
- Nir Hacohen
Affiliations
- Bo Li
- Harvard University Virology Program, Harvfvard Medical School
- Sara M. Clohisey
- Roslin Institute, University of Edinburgh
- Bing Shao Chia
- Harvard University Virology Program, Harvfvard Medical School
- Bo Wang
- Roslin Institute, University of Edinburgh
- Ang Cui
- Broad Institute of MIT and Harvard
- Thomas Eisenhaure
- Broad Institute of MIT and Harvard
- Lawrence D. Schweitzer
- Broad Institute of MIT and Harvard
- Paul Hoover
- Broad Institute of MIT and Harvard
- Nicholas J. Parkinson
- Roslin Institute, University of Edinburgh
- Aharon Nachshon
- School of Molecular Cell Biology and Biotechnology, Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University
- Nikki Smith
- Roslin Institute, University of Edinburgh
- Tim Regan
- Roslin Institute, University of Edinburgh
- David Farr
- Roslin Institute, University of Edinburgh
- Michael U. Gutmann
- School of informatics, University of Edinburgh
- Syed Irfan Bukhari
- Center for Cancer Research, Massachusetts General hospital, Harvard Medical School
- Andrew Law
- Roslin Institute, University of Edinburgh
- Maya Sangesland
- The Ragon Institute of Massachusetts General Hospital, MIT and Harvard University
- Irit Gat-Viks
- Broad Institute of MIT and Harvard
- Paul Digard
- Roslin Institute, University of Edinburgh
- Shobha Vasudevan
- Center for Cancer Research, Massachusetts General hospital, Harvard Medical School
- Daniel Lingwood
- The Ragon Institute of Massachusetts General Hospital, MIT and Harvard University
- David H. Dockrell
- MRC Center for Inflammation Research, University of Edinburgh
- John G. Doench
- Broad Institute of MIT and Harvard
- J. Kenneth Baillie
- Roslin Institute, University of Edinburgh
- Nir Hacohen
- Broad Institute of MIT and Harvard
- DOI
- https://doi.org/10.1038/s41467-019-13965-x
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 18
Abstract
Here, Li et al. perform a genome-wide CRISPR screen to identify host dependency factors for influenza A virus infection and show that the host mRNA cap methyltransferase CMTR1 is important for viral cap snatching and that it affects expression of antiviral genes.
