Frontiers in Plant Science (Jan 2023)
Exogenous spermidine improved drought tolerance in Ilex verticillata seedlings
- Xiaoting Xie,
- Xiaoting Xie,
- Yujie Gu,
- Yujie Gu,
- Weili Wang,
- Weili Wang,
- Farhat Abbas,
- Farhat Abbas,
- Sini Qin,
- Sini Qin,
- Siyi Fu,
- Siyi Fu,
- Jiaqi Mei,
- Jiaqi Mei,
- Jiayan Wang,
- Jiayan Wang,
- Dexuan Ma,
- Dexuan Ma,
- Guangchao Wen,
- Guangchao Wen,
- Ying Yang,
- Ying Yang,
- Anket Sharma,
- Anket Sharma,
- Xiaofei Wang,
- Xiaofei Wang,
- Daoliang Yan,
- Daoliang Yan,
- Bingsong Zheng,
- Bingsong Zheng,
- Yi He,
- Yi He,
- Huwei Yuan,
- Huwei Yuan
Affiliations
- Xiaoting Xie
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Xiaoting Xie
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Yujie Gu
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Yujie Gu
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Weili Wang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Weili Wang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Farhat Abbas
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Farhat Abbas
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Sini Qin
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Sini Qin
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Siyi Fu
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Siyi Fu
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Jiaqi Mei
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Jiaqi Mei
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Jiayan Wang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Jiayan Wang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Dexuan Ma
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Dexuan Ma
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Guangchao Wen
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Guangchao Wen
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Ying Yang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Ying Yang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Anket Sharma
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Anket Sharma
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Xiaofei Wang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Xiaofei Wang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Daoliang Yan
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Daoliang Yan
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Bingsong Zheng
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Bingsong Zheng
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Yi He
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Yi He
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Huwei Yuan
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Hangzhou, China
- Huwei Yuan
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agricultural and Forestry University, Hangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2023.1065208
- Journal volume & issue
-
Vol. 14
Abstract
Winterberry (Ilex verticillata (L.) A. Gray) is a recently introduced ornamental tree species in China that has not been closely investigated for its drought resistance. In this study, we used two-year-old cuttings from I. verticillata (L.) A. Gray and two representative varieties derived from it, I. verticillata ‘Oosterwijk’ and I. verticillata ‘Jim Dandy’, as materials to investigate how this plant responds to drought stress and whether exogenous spermidine (SPD) can alleviate the negative effects caused by drought stress. The results showed that as the degree of drought stress increased, the leaves of winterberry seedlings became chlorotic, and their edges became dry. Similarly, the relative water content, specific leaf weight, chlorophyll content, leaf nitrogen content, net photosynthetic rate, stomatal conductance and transpiration rate were significantly reduced, whereas the content of malondialdehyde continuously increased with the degree of drought stress. The activities of superoxide dismutase, peroxidase, and catalase increased under moderate drought stress and then decreased under severe drought stress. The levels of soluble sugar and abscisic acid continued to increase, while those of auxin and gibberellic acid decreased. When compared with individual drought stress, an increase in the amount of external SPD clearly alleviated the effect of drought stress on winterberry seedlings. The combined phenotypes and physiological indices of the winterberry leaves under drought stress conditions revealed that the drought resistance of the native species was significantly higher than its two varieties. This finding serves as an important theoretical foundation for the popularization and application of I. verticillata (L.) A. Gray and the two varieties.
Keywords
