Nature Communications (Nov 2018)

Chromatin conformation analysis of primary patient tissue using a low input Hi-C method

  • Noelia Díaz,
  • Kai Kruse,
  • Tabea Erdmann,
  • Annette M. Staiger,
  • German Ott,
  • Georg Lenz,
  • Juan M. Vaquerizas

DOI
https://doi.org/10.1038/s41467-018-06961-0
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

Read online

Chromatin conformation studies are limited by the large amounts of starting material required to perform current protocols. Here the authors present Low-C, a Hi-C method for low amounts of input material and produce Low-C maps from primary B-cells of a diffuse large B-cell lymphoma patient, demonstrating the suitability of Low-C to analyse rare cell populations.