Antimicrobial resistance of Escherichia coli isolated from cattle carcasses and feces in Center of Mexico

Abstract

Escherichia coli is an important microorganism as an intestinal microbiota of animals and humans, so its presence in food serves as an indicator of possible fecal contamination; some strains can cause disease, mainly due to the consumption of water and animal food contaminated. The objective of this study was to detemine the resistance to antimicrobials and the genetic character of E. coli present in the carcasses and feces of bovines killed in slaugtherhouses, and to know the epidemiological panorama in Mexico. The study was carried out in 32 strains in three municipal slaugtherhouses (A, B and C) obtained from bovines in Central Mexico; their resistance profile and their genetic relationship between the different isolates were analyzed by genotyping with the enzyme XbaI-PFGE; The dendrogram was constructed using the coefficient of similarity of Dice with a tolerance of 1.5 %. It is observed that 75 % (24/32) of the isolates show resistance to some antibiotic, 84.3 % (27/32) have an intermediate profile and 12.5 % (4/32) are sensitive to all antibiotics, the 28.1 % (9/32), were MDR; 27 PFGE and pulsetypes will be identified; 7 clusters were formed with 2 or more isolates (A-F and I) and two integrated with a strain (G and H). This study shows a diversity antimicrobial resistance present in cattle carcasses and feces in Mexico, which is a risk factor and a public health problem.

Keywords

• escherichia coli
• fecal contamination
• carcass
• food contamination