Frontiers in Dental Medicine (Jul 2023)

Lactobacillus acidophilus impairs the establishment of pathogens in a subgingival multispecies biofilm

  • Manuela Rocha Bueno,
  • Gustavo Dudu-Silva,
  • Tatiane Tiemi Macedo,
  • Aline Paim de Abreu Paulo Gomes,
  • Arthur Rodrigues Oliveira Braga,
  • Lucas Daylor Aguiar Silva,
  • Bruno Bueno-Silva,
  • Bruno Bueno-Silva

DOI
https://doi.org/10.3389/fdmed.2023.1212773
Journal volume & issue
Vol. 4

Abstract

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The present study evaluated the antibiofilm effects of Lactobacillus acidophilus within a subgingival multispecies biofilm. Lactobacillus acidophilus (La5) at 1 × 102, 1 × 104, and 1 × 106 were included at the beginning of biofilm formation, which lasted 7 days. The biofilms comprised 33 periodontitis-related bacterial species and the Calgary Biofilm device was used. At the end, DNA–DNA hybridization (checkerboard) was performed. A Kruskal–Wallis test followed by a Dunn post hoc test were performed (p ≤ 0.05). La5 at 1 × 104 and 1 × 106 reduced the total counts of biofilm and the proportions of red and green complexes when compared to the control biofilm without La5 (p ≤ 0.05). La5 at 1 × 104 increased the proportions of Actinomyces complex compared to the controls (p ≤ 0.05). Both La5 at 1 × 104 and 1 × 106 decreased levels of 20 and 14 distinct species, respectively, including Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum polymorphum, and Parvimonas micra compared to the control (p ≤ 0.05). Only La5 at 1 × 104 reduced the levels of Tannerella forsythia, Fusobacterium periodonticum, and Aggregatibacter actinomycetencomytans compared to the control (p ≤ 0.05). L. acidophilus inhibited establishing periodontic pathogens from red complex such as P. gingivalis and T. forsythia in a subgingival multispecies biofilm.

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