Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein

Yong Lei; Xiaotian Zhang; Jianzhong Su; Mira Jeong; Michael C. Gundry; Yung-Hsin Huang; Yubin Zhou; Wei Li; Margaret A. Goodell

doi:10.1038/ncomms16026

Nature Communications (Jul 2017)

Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein

Yong Lei,
Xiaotian Zhang,
Jianzhong Su,
Mira Jeong,
Michael C. Gundry,
Yung-Hsin Huang,
Yubin Zhou,
Wei Li,
Margaret A. Goodell

Affiliations

Yong Lei: Department of Molecular and Human Genetics, Center for Cell and Gene Therapy, Baylor College of Medicine
Xiaotian Zhang: Department of Molecular and Human Genetics, Center for Cell and Gene Therapy, Baylor College of Medicine
Jianzhong Su: Dan L. Duncan Cancer Center and Department of Molecular and Cellular Biology, Baylor College of Medicine
Mira Jeong: Department of Molecular and Human Genetics, Center for Cell and Gene Therapy, Baylor College of Medicine
Michael C. Gundry: Department of Molecular and Human Genetics, Center for Cell and Gene Therapy, Baylor College of Medicine
Yung-Hsin Huang: Stem Cells and Regenerative Medicine Center, Baylor College of Medicine
Yubin Zhou: Department of Medical Physiology, Center for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University
Wei Li: Stem Cells and Regenerative Medicine Center, Baylor College of Medicine
Margaret A. Goodell: Department of Molecular and Human Genetics, Center for Cell and Gene Therapy, Baylor College of Medicine

DOI: https://doi.org/10.1038/ncomms16026
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 10

Abstract

Read online

Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal