Multimodal two-photon imaging using a second harmonic generation-specific dye

Mutsuo Nuriya; Shun Fukushima; Atsuya Momotake; Takanori Shinotsuka; Masato Yasui; Tatsuo Arai

doi:10.1038/ncomms11557

Nature Communications (May 2016)

Multimodal two-photon imaging using a second harmonic generation-specific dye

Mutsuo Nuriya,
Shun Fukushima,
Atsuya Momotake,
Takanori Shinotsuka,
Masato Yasui,
Tatsuo Arai

Affiliations

Mutsuo Nuriya: Department of Pharmacology School of Medicine, Keio University
Shun Fukushima: Graduate School of Pure and Applied Sciences and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba
Atsuya Momotake: Graduate School of Pure and Applied Sciences and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba
Takanori Shinotsuka: Department of Pharmacology School of Medicine, Keio University
Masato Yasui: Department of Pharmacology School of Medicine, Keio University
Tatsuo Arai: Graduate School of Pure and Applied Sciences and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba

DOI: https://doi.org/10.1038/ncomms11557
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

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Current dyes for second harmonic generation (SHG) imaging strongly fluoresce, limiting their application. Here the authors develop a SHG-specific dye, Ap3, that partitions into cell membranes, displays sensitivity to membrane potential and has virtually no fluorescence emission at SHG imaging wavelengths.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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