Comparison between MPO, CD177 and NF-κB Gene Expression of Septic Rats Treated with Mentha longifolia Essential Oils

Abstract

Background and Aim: Incidences range of sepsis-associated liver dysfunction and lung injury are observed in patients with sepsis. Mentha longifolia as a native Iranian plant have many properties such as antimicrobial and antioxidant activity. In this regard, the anti-inflammatory effect of Mentha longifolia essential oil (E.O) on liver and lung injury induced by sepsis was investigated. Materials and Methods: Gene expression of three genes (MPO, CD177 and NF-κB) as inflammatory factors was evaluated by real-time PCR in four experimental groups (Laparotomy (LAP), cecal ligation and puncture (CLP) and essential oil groups at 50 and 100 mg/kg body weight doses). The treatment was carried out orally for two weeks before CLP surgery. 24 hours after CLP, samples were immediately transferred to -80ºC to maintain for further experiments. Results: Expression of genes increased in the CLP group as compared to the LAP group. Treatments of rats with essential oil have been effective in decreasing genes expression levels in two dosages at two tissues. Conclusion: Our results demonstrated that M. longifolia essential oil treatment could protect against sepsis-induced liver and lung injury by reducing inflammatory factor.

Keywords

• myeloperoxidase, gene expression, sepsis