Nature Communications (Aug 2018)

A novel probe to assess cytosolic entry of exogenous proteins

  • Qiao Lu,
  • Jeff E. Grotzke,
  • Peter Cresswell

DOI
https://doi.org/10.1038/s41467-018-05556-z
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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The mechanism of protein dislocation into the cytosol during antigen cross-presentation is poorly understood. Here the authors engineer a dislocation reporter fusing a glycosylated luciferase variant to the Fc region of IgG1, and find that dislocation is the rate limiting step in cross-presentation.