A nanometric DNA-zwitterionic phospholipid unilamellar vesicle formulation compacted for gene delivery: Adiabatic differential scanning microcalorimetric study

Abstract

Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper zwitterionic lipid, the latter being applied for the stabilization of the whole complex. However, besides problematic steps during their preparation, cationic lipids are toxic for cells. Present work describes some energetics issues pertinent to preparation and use of neutral lipid-DNA self-assemblies, thus avoiding toxicity of lipoplexes. Differential scanning calorimetry data showed stabilization of polynucleotide helix upon its interaction with liposomes in the presence of divalent metal cations. It is thus possible to suggest this selfassembly as an improved formulation for use in gene delivery.

Keywords

• DNA-liposome self-assembly
• thermodynamics
• divalent metal cation
• differential scanning microcalorimetry
• non-viral gene delivery