Plants (Nov 2019)

Chemical Composition, Enantiomeric Distribution, and Sensory Evaluation of the Essential Oils Distilled from the Ecuadorian Species <i>Myrcianthes myrsinoides</i> (Kunth) Grifo and <i>Myrcia mollis</i> (Kunth) DC. (Myrtaceae)

  • Mayra Montalván,
  • Manuel Alejandro Peñafiel,
  • Jorge Ramírez,
  • Nixon Cumbicus,
  • Nicole Bec,
  • Christian Larroque,
  • Carlo Bicchi,
  • Gianluca Gilardoni

DOI
https://doi.org/10.3390/plants8110511
Journal volume & issue
Vol. 8, no. 11
p. 511

Abstract

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The essential oils of Myrcianthes myrsinoides and Myrcia mollis, belonging to the Myrtaceae family, were obtained by steam distillation. They were analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography-flame ionization detector (GC-FID), enantioselective gas chromatography, and gas chromatography-olfactometry (GC-O). A total of 58 compounds for Myrcianthes myrsinoides essential oil (EO) and 22 compounds for Myrcia mollis EO were identified and quantified by GC-MS with apolar and polar columns (including undetermined components). Major compounds (>5.0%) were limonene (5.3%−5.2%), 1,8-cineole (10.4%−11.6%), (Z)-caryophyllene (16.6%−16.8%), trans-calamenene (15.9%−14.6%), and spathulenol (6.2%−6.5%). The enantiomeric excess of eight chiral constituents was determined, being (+)-limonene and (+)-germacrene D enantiomerically pure. Eight components were identified as determinant in the aromatic profile: α-pinene, β-pinene, (+)-limonene, γ-terpinene, terpinolene, linalool, β-elemene and spathulenol. For M. mollis, the major compounds (>5.0%) were α-pinene (29.2%−27.7%), β-pinene (31.3%−30.0%), myrcene (5.0%−5.2%), 1,8-cineole (8.5%−8.7%), and linalool (7.7%−8.2%). The enantiomeric excess of five chiral constituents was determined, with (S)-α-pinene and (+)-germacrene D enantiomerically pure. The metabolites β-pinene, 1,8-cineole, γ-terpinene, terpinolene, linalool, and (E)-β-caryophyllene were mainly responsible for the aroma of the EO. Finally, the M. myrsinoides essential oil has an inhibitory activity for cholinesterase enzymes (IC50 of 78.6 μg/mL and 18.4 μg/mL vs. acethylcholinesterase (AChE) and butyrylcholinesterase (BChE) respectively). This activity is of interest to treat Alzheimer’s disease.

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