A homologue of the Parkinson’s disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover

Egon Deyaert; Lina Wauters; Giambattista Guaitoli; Albert Konijnenberg; Margaux Leemans; Susanne Terheyden; Arsen Petrovic; Rodrigo Gallardo; Laura M. Nederveen-Schippers; Panagiotis S. Athanasopoulos; Henderikus Pots; Peter J. M. Van Haastert; Frank Sobott; Christian Johannes Gloeckner; Rouslan Efremov; Arjan Kortholt; Wim Versées

doi:10.1038/s41467-017-01103-4

Nature Communications (Oct 2017)

A homologue of the Parkinson’s disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover

Egon Deyaert,
Lina Wauters,
Giambattista Guaitoli,
Albert Konijnenberg,
Margaux Leemans,
Susanne Terheyden,
Arsen Petrovic,
Rodrigo Gallardo,
Laura M. Nederveen-Schippers,
Panagiotis S. Athanasopoulos,
Henderikus Pots,
Peter J. M. Van Haastert,
Frank Sobott,
Christian Johannes Gloeckner,
Rouslan Efremov,
Arjan Kortholt,
Wim Versées

Affiliations

Egon Deyaert: VIB-VUB Center for Structural Biology
Lina Wauters: VIB-VUB Center for Structural Biology
Giambattista Guaitoli: German Center for Neurodegenerative Diseases (DZNE)
Albert Konijnenberg: Department of Chemistry, Biomolecular & Analytical Mass Spectrometry group, University of Antwerp
Margaux Leemans: VIB-VUB Center for Structural Biology
Susanne Terheyden: Department of Cell Biochemistry, University of Groningen
Arsen Petrovic: Department of Mechanistic Cell Biology, Max-Planck Institute of Molecular Physiology
Rodrigo Gallardo: VIB Center for Brain & Disease Research
Laura M. Nederveen-Schippers: Department of Cell Biochemistry, University of Groningen
Panagiotis S. Athanasopoulos: Department of Cell Biochemistry, University of Groningen
Henderikus Pots: Department of Cell Biochemistry, University of Groningen
Peter J. M. Van Haastert: Department of Cell Biochemistry, University of Groningen
Frank Sobott: Department of Chemistry, Biomolecular & Analytical Mass Spectrometry group, University of Antwerp
Christian Johannes Gloeckner: German Center for Neurodegenerative Diseases (DZNE)
Rouslan Efremov: VIB-VUB Center for Structural Biology
Arjan Kortholt: Department of Cell Biochemistry, University of Groningen
Wim Versées: VIB-VUB Center for Structural Biology

DOI: https://doi.org/10.1038/s41467-017-01103-4
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

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The Parkinson’s disease‐associated LRRK2 protein is a multidomain Roco protein with GTPase activity. Here the authors use a multidisciplinary approach to characterize the GTPase mechanism of a homologous bacterial Roco protein and give mechanistic insights into disease-causing LRRK2 mutations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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