Artificial Cells, Nanomedicine, and Biotechnology (Dec 2024)

A consortium of Hordeum vulgare and gut microbiota against non-alcoholic fatty liver disease via data-driven analysis

  • Su-Been Lee,
  • Haripriya Gupta,
  • Byeong-Hyun Min,
  • Raja Ganesan,
  • Satya Priya Sharma,
  • Sung-Min Won,
  • Jin-Ju Jeong,
  • Min-Gi Cha,
  • Goo-Hyun Kwon,
  • Min-Kyo Jeong,
  • Ji-Ye Hyun,
  • Jung-A Eom,
  • Hee-Jin Park,
  • Sang-Jun Yoon,
  • Sang Youn Lee,
  • Mi-Ran Choi,
  • Dong Joon Kim,
  • Ki-Kwang Oh,
  • Ki-Tae Suk

DOI
https://doi.org/10.1080/21691401.2024.2347380
Journal volume & issue
Vol. 52, no. 1
pp. 250 – 260

Abstract

Read online

AbstractDespite many recent studies on non-alcoholic fatty liver disease (NAFLD) therapeutics, the optimal treatment has yet to be determined. In this unfinished project, we combined secondary metabolites (SMs) from the gut microbiota (GM) and Hordeum vulgare (HV) to investigate their combinatorial effects via network pharmacology (NP). Additionally, we analyzed GM or barley – signalling pathways – targets – metabolites (GBSTMs) in combinatorial perspectives (HV, and GM). A total of 31 key targets were analysed via a protein-protein interaction (PPI) network, and JUN was identified as the uppermost target in NAFLD. On a bubble plot, we revealed that apelin signalling pathway, which had the lowest enrichment factor antagonize NAFLD. Holistically, we scrutinized GBSTM to identify key components (GM, signalling pathways, targets, and metabolites) associated with the Apelin signalling pathway. Consequently, we found that the primary GMs (Eubacterium limosum, Eggerthella sp. SDG-2, Alistipes indistinctus YIT 12060, Odoribacter laneus YIT 12061, Paraprevotella clara YIT 11840, Paraprevotella xylaniphila YIT 11841) to ameliorate NAFLD. The molecular docking test (MDT) suggested that tryptanthrin-JUN is an agonist, conversely, dihydroglycitein-HDAC5, 1,3-diphenylpropan-2-ol-NOS1, and (10[(Acetyloxy)methyl]-9-anthryl)methyl acetate-NOS2, which are antagonistic conformers in the apelin signalling pathway. Overall, these results suggest that combination therapy could be an effective strategy for treating NAFLD.

Keywords