Stimulation of cholesterol 7α-hydroxylase by phenobarbital in two strains of rats

S. Shefer; S. Hauser; E.H. Mosbach

Journal of Lipid Research (Jan 1972)

Stimulation of cholesterol 7α-hydroxylase by phenobarbital in two strains of rats

S. Shefer,
S. Hauser,
E.H. Mosbach

Affiliations

S. Shefer: Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York 10016
S. Hauser: Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York 10016
E.H. Mosbach: Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York 10016

Journal volume & issue: Vol. 13, no. 1
pp. 69 – 70

Abstract

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The effect of phenobarbital administration on the in vitro activity of cholesterol 7α-hydroxylase was investigated in two strains of rats. In rats of the Wistar strain the daily injection of phenobarbital (100 mg/kg per day ip for 5 days) produced a 33% increase in hepatic microsomal protein and a sixfold stimulation of specific activity of the enzyme. In rats of the Charles River colony (Sprague-Dawley derived) identical treatment with phenobarbital resulted in a 45% increase in hepatic microsomal protein and no change in the specific activity of cholesterol 7α-hydroxylase. Plasma phenobarbital concentrations were three to four times greater in the Wistar rats, suggesting that these strains differ also in their capacity to metabolize phenobarbital.

microsomal enzyme

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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