Mapping of wheat stripe rust resistance gene Yr041133 by BSR-Seq analysis

Yahui Li; Ruiming Lin; Jinghuang Hu; Xiaohan Shi; Dan Qiu; Peipei Wu; Gebremedhin Habteab Goitom; Siqi Wang; Hongjun Zhang; Li Yang; Hongwei Liu; Qiuhong Wu; Jingzhong Xie; Yang Zhou; Zhiyong Liu; Hongjie Li

Crop Journal (Apr 2022)

Mapping of wheat stripe rust resistance gene Yr041133 by BSR-Seq analysis

Yahui Li,
Ruiming Lin,
Jinghuang Hu,
Xiaohan Shi,
Dan Qiu,
Peipei Wu,
Gebremedhin Habteab Goitom,
Siqi Wang,
Hongjun Zhang,
Li Yang,
Hongwei Liu,
Qiuhong Wu,
Jingzhong Xie,
Yang Zhou,
Zhiyong Liu,
Hongjie Li

Affiliations

Yahui Li: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ruiming Lin: State Key Laboratory for Biology of Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Jinghuang Hu: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xiaohan Shi: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Dan Qiu: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Peipei Wu: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Gebremedhin Habteab Goitom: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Siqi Wang: College of Humanities and Development Studies, China Agricultural University, Beijing 100083, China
Hongjun Zhang: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Li Yang: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hongwei Liu: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Qiuhong Wu: Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
Jingzhong Xie: Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
Yang Zhou: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhiyong Liu: Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; Corresponding authors.
Hongjie Li: The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.

Journal volume & issue: Vol. 10, no. 2
pp. 447 – 455

Abstract

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Puccinia striiformis Westend. f. sp. tritici (Pst) pathotype CYR34 is widely virulent and prevalent in China. Here, we report identification of a strpie rust resistance (Yr) gene, designated Yr041133, in winter wheat line 041133. This line produced a hypersensitive reaction to CYR34 and conferred resistance to 13 other pathotypes. Resistance to CYR34 in line 041133 was controlled by a single dominant gene. Bulked segregant RNA sequencing (BSR-Seq) was performed on a pair of RNA bulks generated by pooling resistant and susceptible recombinant inbred lines. Yr041133 was mapped to a 1.7 cM genetic interval on the chromosome arm 7BL that corresponded to a 0.8 Mb physical interval (608.9–609.7 Mb) in the Chinese Spring reference genome. Based on its unique physical location Yr041133 differred from the other Yr genes on this chromosome arm.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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