Anti-inflammatory, analgesic and molecular docking studies of Lanostanoic acid 3-O-α-D-glycopyranoside isolated from Helichrysum stoechas

Abstract

Helichrysum stoechas has been conventionally used as herbal tea due to its anti-inflammatory, antioxidant, antimicrobial and diuretic activities. Ethanolic extract of the aerial parts of the plant (HSE) afforded a lanostane triterpenoid glycoside. The isolated compound was characterized as Lanostan-3β-olyl-26-oic acid 3-O-α-D-glycopyranoside (HS-01) with the help of UV, IR, 1H, 13C NMR and MS spectroscopic techniques. HSE (at 100 and 200 mg/kg doses) and the isolated compound, HS-01 (at 10 mg/kg dose) has been investigated for anti-inflammatory and analgesic activities against chemically challenged experimental animal. Both the HSE as well as HS-01 showed a substantial decline in paw volume when compared with the relevant control groups (p < 0.01 & p < 0.001). The HSE and HS-01 also confirms a significant prolongation of the paw licking or jumping towards the Eddy’s hot plate and reduction in quantity of writhes after the introduction of acetic acid in mice (p < 0.01 & p < 0.001). In order to have a better understanding of the binding interactions of HS-01 at molecular level, docking studies were performed with various macromolecular drug targets using AutoDock 4.2 and AutoDock Vina 1.1. Both programs predicted Galectin-3 as most favorable target for HS-01 followed by iNOS, whereas TNFα and COX-2 were among less favorable. Therefore, HS-01 could be developed as suitable therapy against inflammation and associated disorders.

Keywords

• Helichrysum stoechas
• Phytochemical
• Lanostane triterpenoids glycosides
• Molecular docking
• Anti-inflammatory