Extraction of Mycosporine-like Amino Acids and Proteins from the Agarophyte <i>Gelidium corneum</i> Using Pulsed Power Techniques
Colin McReynolds,
Amandine Adrien,
Antoine Silvestre de Ferron,
Nadia Boussetta,
Nabil Grimi,
Laurent Pecastaing,
Susana C. M. Fernandes
Affiliations
Colin McReynolds
IPREM—Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials, E2S UPPA: Energy Environment Solutions, Université de Pau et des Pays de l’Adour, CNRS—Centre National de la Recherche Scientifique, 64600 Anglet, France
Amandine Adrien
IPREM—Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials, E2S UPPA: Energy Environment Solutions, Université de Pau et des Pays de l’Adour, CNRS—Centre National de la Recherche Scientifique, 64600 Anglet, France
Antoine Silvestre de Ferron
Laboratoire des Sciences de l’Ingénieur Appliquées à la Mécanique et au Génie Électrique—Fédération IPRA, Université de Pau et des Pays de l’Adour/E2S UPPA, EA4581, 64000 Pau, France
Nadia Boussetta
TIMR (Integrated Transformations of Renewable Matter), ESCOM, Université de Technologie de Compiègne, Sorbonne Universités, Centre de Recherche Royallieu, CEDEX CS 60319, 60203 Compiègne, France
Nabil Grimi
TIMR (Integrated Transformations of Renewable Matter), ESCOM, Université de Technologie de Compiègne, Sorbonne Universités, Centre de Recherche Royallieu, CEDEX CS 60319, 60203 Compiègne, France
Laurent Pecastaing
IPREM—Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials, E2S UPPA: Energy Environment Solutions, Université de Pau et des Pays de l’Adour, CNRS—Centre National de la Recherche Scientifique, 64600 Anglet, France
Susana C. M. Fernandes
IPREM—Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials, E2S UPPA: Energy Environment Solutions, Université de Pau et des Pays de l’Adour, CNRS—Centre National de la Recherche Scientifique, 64600 Anglet, France
Gelidium corneum (syn. sesquipedale) is an industrially and ecologically important species of red alga used for the production of high-quality agar. However, the species is also of growing interest for the production of other valuable compounds, such as mycosporine-like amino acids (MAAs), with potential cosmeceutical and biomedical applications. Novel methods using two pulsed power techniques, high-voltage electrical discharges (HVED) and pulsed electrical fields (PEF), were evaluated for efficacy of MAA extraction. Algal suspensions were prepared at two ratios (1:20 and 1:40 w:v). Four different extraction protocols were compared: (i) high-voltage electrical discharges, (ii) pulsed electric fields, (iii) maceration at room temperature, and (iv) maceration at 50 °C. The algae were treated in three states: freshly harvested, dried, and powdered. HVED and PEF treatments were effective when performed on fresh algae, and in particular the HVED treatment resulted in yields of MAAs twenty times higher than the control: 0.81 ± 0.05 mg/gDry Weight (DW) vs. 0.037 ± 0.002 mg/gDW. This effect was not observed to the same extent when the algae were dried or powdered, although HVED remained the most selective method overall.
