Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach

Abstract

Abstract Background Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.

Keywords

• Cyclospora
• qPCR
• Mutation scanning-based sequencing
• Non-human primates
• Zoonosis