OPTIMIZATION OF SOLID-PHASE SYNTHESIS OF POLYAMIDE NUCLEIC ACID MIMETICS WITH APPLICATION OF MALDI-TOF MASS-SPECTROMETRY

Abstract

The solid-phase synthesis of a tetrameric model sequence of polyamide nucleic acid mimetics (PANAM) incorporating terminal chiral and charged unit was performed. As a result during the synthesis and cleavage from the resin the tendency of PANAM with the N-terminal negatively charged monomer to cyclization with simultaneous N-acyl transfer of carboxyethyl base residue was found. Application of MALDI-TOF mass - spectrometry allowed us to find suitable conditions for obtaining PANAM oligomers without the significant amount of by-products.

Keywords

• полиамидные миметики нуклеиновых кислот
• пептидно-нуклеиновые кислоты (пнк)
• твердофазный синтез
• maldi-tof-масс-спектрометрия
• polyamide nucleic acid mimetics (panam)
• peptide nucleic acids (pna)
• solid phase synthesis
• maldi-tof mass-spectrometry