Frontiers in Microbiology (Sep 2019)

Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field

  • Yashpal Singh Malik,
  • Atul Kumar Verma,
  • Naveen Kumar,
  • Nadia Touil,
  • Kumaragurubaran Karthik,
  • Ruchi Tiwari,
  • Durlav Prasad Bora,
  • Kuldeep Dhama,
  • Souvik Ghosh,
  • Maged Gomaa Hemida,
  • Maged Gomaa Hemida,
  • Ahmed S. Abdel-Moneim,
  • Ahmed S. Abdel-Moneim,
  • Krisztián Bányai,
  • Anastasia N. Vlasova,
  • Nobumichi Kobayashi,
  • Raj Kumar Singh

DOI
https://doi.org/10.3389/fmicb.2019.01957
Journal volume & issue
Vol. 10

Abstract

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The applications of correct diagnostic approaches play a decisive role in timely containment of infectious diseases spread and mitigation of public health risks. Nevertheless, there is a need to update the diagnostics regularly to capture the new, emergent, and highly divergent viruses. Acute gastroenteritis of viral origin has been identified as a significant cause of mortality across the globe, with the more serious consequences seen at the extremes of age groups (young and elderly) and immune-compromised individuals. Therefore, significant advancements and efforts have been put in the development of enteric virus diagnostics to meet the WHO ASSURED criteria as a benchmark over the years. The Enzyme-Linked Immunosorbent (ELISA) and Polymerase Chain Reaction (PCR) are the basic assays that provided the platform for development of several efficient diagnostics such as real-time RT-PCR, loop-mediated isothermal amplification (LAMP), polymerase spiral reaction (PSR), biosensors, microarrays and next generation sequencing. Herein, we describe and discuss the applications of these advanced technologies in context to enteric virus detection by delineating their features, advantages and limitations.

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