Validitas Multiplex Real Time Polymerase Chain Reaction untuk Diagnosis Limfadenitis Tuberkulosis pada Spesimen Blok Parafin

Abstract

Tuberculous lymphadenitis is the most form of extra pulmonary tuberculous. The diagnosis of tuberculous lymphadenitis based on histopathology as a gold standard. Histopathology examination could not inform the etiology lymphadenitis whether Mycobacterium tuberculous or Mycobacterium nontuberculous of which the treatment regiment is different. The multiplex real time polymerase chain reaction can detect Mycobacterium tuberculosis or Mycobacterium nontuberculosis from formalin fixed paraffin embedded. The study with cross sectional design to determine validity multiplex real time polymerase chain reaction on tuberculous lymphadenitis from formalin fixed paraffin embedded, was done June 2012˗˗June 2013 at Dr. Hasan Sadikin Hospital Bandung. Objects consisted of 40 formalin fixed paraffin embedded tuberculous lymphadenitis and 22 control group. The multiplex real time polymerase chain reaction use insertion sequence IS6110 and Mycobacterium protein tuberculosis MPB64 gen for detect deoxyribonucleic acid Mycobacterium tuberculosis and 16S ribosomal ribonucleic acid gen for Mycobacterium nontuberculosis. The results of validity test sensitivity 75%, specificity 77%, positive predictive value 86,6%, negative predictive value 63%, and accuracy 75,8%. This examination can be used for confirmation of tuberculous lymphadenitis from formalin fixed paraffin embedded. Conclussion: The multiplex real time polymerase chain reaction gave a intermediate validity test of the diagnosis tuberculous lymphadenitis from formalin fixed paraffin embedded.

Keywords

• deoxyribonucleic acid
• multiplex real time polymerase chain reaction
• Mycobacterium tuberculous
• tuberculous lymphadenitis