Recent Molecular Characterization of Porcine Rotaviruses Detected in China and Their Phylogenetic Relationships with Human Rotaviruses
Mengli Qiao,
Meizhen Li,
Yang Li,
Zewei Wang,
Zhiqiang Hu,
Jie Qing,
Jiapei Huang,
Junping Jiang,
Yaqin Jiang,
Jinyong Zhang,
Chunliu Gao,
Chen Yang,
Xiaowen Li,
Bin Zhou
Affiliations
Mengli Qiao
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China
Meizhen Li
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China
Yang Li
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Zewei Wang
Beef Cattle Industry Development Center, Fangshan 033100, China
Zhiqiang Hu
College of Animal Science, Xichang University, Xichang 615012, China
Jie Qing
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Jiapei Huang
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Junping Jiang
China Agriculture Research System-Yangling Comprehensive Test Station, Xianyang 712100, China
Yaqin Jiang
China Agriculture Research System-Yangling Comprehensive Test Station, Xianyang 712100, China
Jinyong Zhang
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Chunliu Gao
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Chen Yang
Shandong Engineering Research Center of Pig and Poultry Health Breeding and Important Disease Purification, Shandong New Hope Liuhe Co., Ltd., Qingdao 266000, China
Xiaowen Li
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China
Bin Zhou
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China
Porcine rotavirus A (PoRVA) is an enteric pathogen capable of causing severe diarrhea in suckling piglets. Investigating the prevalence and molecular characteristics of PoRVA in the world, including China, is of significance for disease prevention. In 2022, a total of 25,768 samples were collected from 230 farms across China, undergoing porcine RVA positivity testing. The results showed that 86.52% of the pig farms tested positive for porcine RVA, with an overall positive rate of 51.15%. Through the genetic evolution analysis of VP7, VP4 and VP6 genes, it was revealed that G9 is the predominant genotype within the VP7 segment, constituting 56.55%. VP4 genotypes were identified as P[13] (42.22%), P[23] (25.56%) and P[7] (22.22%). VP6 exhibited only two genotypes, namely I5 (88.81%) and I1 (11.19%). The prevailing genotype combination for RVA was determined as G9P[23]I5. Additionally, some RVA strains demonstrated significant homology between VP7, VP4 and VP6 genes and human RV strains, indicating the potential for human RV infection in pigs. Based on complete genome sequencing analysis, a special PoRVA strain, CHN/SD/LYXH2/2022/G4P[6]I1, had high homology with human RV strains, revealing genetic reassortment between human and porcine RV strains in vivo. Our data indicate the high prevalence, major genotypes, and cross-species transmission of porcine RVA in China. Therefore, the continuous monitoring of porcine RVA prevalence is essential, providing valuable insights for virus prevention and control, and supporting the development of candidate vaccines against porcine RVA.
