Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles

Ling Zhi Wu; Ling Zhi Wu; Yuan Ye; Zhi Xuan Wang; Die Ma; Li Li; Guo Hao Xi; Bi Qing Bao; Li Xing Weng

doi:10.3389/fbioe.2021.690747

Frontiers in Bioengineering and Biotechnology (Jun 2021)

Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles

Ling Zhi Wu,
Ling Zhi Wu,
Yuan Ye,
Zhi Xuan Wang,
Die Ma,
Li Li,
Guo Hao Xi,
Bi Qing Bao,
Li Xing Weng

Affiliations

Ling Zhi Wu: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Ling Zhi Wu: College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, China
Yuan Ye: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Zhi Xuan Wang: College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, China
Die Ma: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Li Li: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Guo Hao Xi: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Bi Qing Bao: Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China
Li Xing Weng: College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, China

DOI: https://doi.org/10.3389/fbioe.2021.690747
Journal volume & issue: Vol. 9

Abstract

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Single-nucleotide polymorphisms (SNPs) are the abundant forms of genetic variations, which are closely associated with serious genetic and inherited diseases, even cancers. Here, a novel SNP detection assay has been developed for single-nucleotide discrimination by nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The SNP of p53 gene mutation in gastric cancer has been successfully detected in the femtomolar concentration by nanopore sensing. The robust biosensing strategy offers a way for solid nanopore sensors integrated with varied nanoparticles to achieve single-nucleotide distinction with high sensitivity and spatial resolution, which promises tremendous potential applications of nanopore sensing for early diagnosis and disease prevention in the near future.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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Abstract

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