Semen Collection, Assessment and Processing for in vitro Fertilization in Dog – a Review

Abstract

The attainment of a feasible in vitro capacitation is clearly dependent on the maintenance of suitable energy levels of mammalian spermatozoa. This is because of the fact that all the sperm changes related to capacitation, such as the increase in tyrosine phosphorylation or changes in motility patterns, are energy-consuming. Canine semen can be capacitated and undergoes acrosome reaction in vitro and spermatozoa are able to fertilize homologous oocytes in in vitro culture conditions. The aim of this paper is to describe the recent research of the authors in the field. Recent and classical reviews and new trends regarding semen prelevation, evaluation and preparation for in vitro fertilization in dog.

Keywords

• capacitation
• cryopreservation
• dog sperm
• evaluation
• prelevation