A simple and high-throughput method for xylem sap collection
Manman Kan,
Kiyoshi Yamazaki,
Toru Fujiwara,
Takehiro Kamiya
Affiliations
Manman Kan
1Department of Applied Biological Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-8657, Japan
Kiyoshi Yamazaki
1Department of Applied Biological Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-8657, Japan
Toru Fujiwara
1Department of Applied Biological Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-8657, Japan
Takehiro Kamiya
1Department of Applied Biological Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-8657, Japan
Xylem is the most important route for root-to-shoot translocation of water, nutrients and signaling molecules. Although a number of studies have been performed using xylem sap, its collection requires special equipment and is usually low throughput. Here, we developed a simple and high-throughput method for the collection of both medium- (<1 ml) and small- (<200 μl) volume xylem sap samples. Using a rice Cd transporter mutant, we demonstrated that our method allows for the effective evaluation of xylem sap Cd concentrations.
