Evaluation of swine fertilisation medium (SFM) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders
P. Fantinati,
A. Zannoni,
C. Bernardini,
M. Forni,
A. Tattini,
E. Seren,
M.L. Bacci
Affiliations
P. Fantinati
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Zannoni
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
C. Bernardini
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M. Forni
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
A. Tattini
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
E. Seren
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
M.L. Bacci
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia 40064 (BO), Italy
In pig production, artificial insemination is widely carried out and the use of fresh diluted semen is predominant. For this reason, there are increasing interests in developing new extenders and in establishing the optimal storage conditions for diluted spermatozoa. In the last few decades, we utilised a homemade diluent (swine fertilisation medium (SFM)) for spermatozoa manipulation and biotechnological application as the production of transgenic pigs utilising the sperm-mediated gene transfer technique. The purpose of the present study is therefore to analyse the ability of SFM, in comparison to four commercial extenders, in preserving the quality of diluted boar semen stored at 16.5°C till 15 days. We utilised some of the main predictive tests as objectively measured motility, acrosome and sperm membrane integrity, high mitochondrial membrane potential and pH. Based on our in vitro study, SFM could be declared as a good long-term extender, able to preserve spermatozoa quality as well as Androhep Enduraguard for up to 6 to 9 days and more.
