First Evidence Indicates the Physiology- and Axial-Myopia-Dependent Profiles of Steroid Hormones in Aqueous Humor
Tiansheng Chou,
Xiaosheng Huang,
Jiawei Liu,
Xinhua Liu,
Kun Zeng,
Zonghui Yan,
Shaoyi Mei,
Liangnan Sun,
Wenqun Xi,
Jinglan Ni,
Jin Zi,
Jun Zhao,
Siqi Liu
Affiliations
Tiansheng Chou
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Xiaosheng Huang
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Jiawei Liu
Department of Proteomics, Beijing Genomics Institute (BGI), Shenzhen 518000, China
Xinhua Liu
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Kun Zeng
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Zonghui Yan
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Shaoyi Mei
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Liangnan Sun
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Wenqun Xi
Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Jinglan Ni
Department of Ophthalmology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, China
Jin Zi
Department of Proteomics, Beijing Genomics Institute (BGI), Shenzhen 518000, China
Jun Zhao
Department of Ophthalmology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, China
Siqi Liu
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
The quantitative level of steroid hormones (SHs) in some body fluids have been accepted for clinical diagnosis, whereas their distribution in aqueous humor (AH) is unknown yet. Herein, a profiling study was conducted with a total of 171 AH and 107 plasma samples using liquid chromatography coupled with tandem mass spectrometry (LC MS/MS). For the first time, six kinds of SHs in AH were quantitatively estimated, and their abundances were ranked at cortisol (F), corticosterone (COR), androstenedione (A2), and 11-deoxycortisol (11DOC). The corresponding abundance of all SHs in AH was significantly lower than those in plasma, while there was a lack of a proportional relationship with the abundance of plasma SHs. Dehydroepiandrosterone sulfate, the most abundant plasma SH, was undetectable in AH, implying that the blood–aqueous barrier might specifically block its transferral. Axial myopia generally results from many factors throughout the entire eye from tissues and molecules; furthermore, the correlation of AH SHs and axial myopia was assessed to look for their indication in such myopia. The panel with five kinds of AH SHs (F, COR, CORT, ALD and A2) was functional as a discriminator for axial myopia and control. The abundance of SHs, therefore, has a specific distribution in AH and can potentially contribute to axial myopia.
