PLoS ONE (Jan 2018)

Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability.

  • Marek Harhala,
  • Jakub Barylski,
  • Kinga Humińska-Lisowska,
  • Dorota Lecion,
  • Jacek Wojciechowicz,
  • Karolina Lahutta,
  • Marta Kuś,
  • Andrew M Kropinski,
  • Sylwia Nowak,
  • Grzegorz Nowicki,
  • Katarzyna Hodyra-Stefaniak,
  • Krystyna Dąbrowska

DOI
https://doi.org/10.1371/journal.pone.0205995
Journal volume & issue
Vol. 13, no. 10
p. e0205995

Abstract

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Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC-ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively).