The Effects of Bee Venom on PLA2, COX-2, iNOS, AA and PG in RAW 264.7 Cells

Abstract

Objectives : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide-induced expression phospholipase A2, cyclooxygenase-2 and inducible nitrogen oxide synthase, and the generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells, a murine macrophage cell line. Methods : The expression of phospholipase A2 , cyclooxygenase and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of arachidonic acid, prostaglandin D2 and E2 was assayed by ELISA method in RAW 264.7 cells. The non-toxic concentrations (0.1 to 5ug/ml) of bee venom determined by MTT assay, were used in this study. Results : 1. Bee venom inhibited lipopolysaccharide-induced expression of phospholipase A2 in a dose dependent manner after 48 hours treatment. 2. Bee venom inhibited lipopolysaccharide-induced expression of cyclooxygenase-2 in a dose dependent manner after 24 and 48 hours treatment. 3. Bee venom inhibited lipopolysaccharide-induced expression of inducible nitrogen oxidesynthase in a dose dependent manner after 48 hours treatment. 4. The generation of arachidonic acid, prostaglandin D2 and E2 was not much affected by the treatment of bee venom on the lipopolysaccharide-induced generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells.

Keywords

• Bee Venom
• Toxic
• PLA2
• COX-2
• iNOS
• AA
• PGD2
• PGE2
• RAW 264.7 Cell