Mapping the sensing spots of aerolysin for single oligonucleotides analysis

Chan Cao; Meng-Yin Li; Nuria Cirauqui; Ya-Qian Wang; Matteo Dal Peraro; He Tian; Yi-Tao Long

doi:10.1038/s41467-018-05108-5

Nature Communications (Jul 2018)

Mapping the sensing spots of aerolysin for single oligonucleotides analysis

Chan Cao,
Meng-Yin Li,
Nuria Cirauqui,
Ya-Qian Wang,
Matteo Dal Peraro,
He Tian,
Yi-Tao Long

Affiliations

Chan Cao: Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology
Meng-Yin Li: Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology
Nuria Cirauqui: Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Ya-Qian Wang: Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology
Matteo Dal Peraro: Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
He Tian: Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology
Yi-Tao Long: Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology

DOI: https://doi.org/10.1038/s41467-018-05108-5
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 9

Abstract

Read online

Nanopores are an emerging powerful single-molecule method of DNA sequencing. Here the authors map the structure of aerolysin for use as a nanopore and show detection of modified and unmodified nucleobases.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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