Scientific Reports (Oct 2022)

Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay

  • Shuvankar Dey,
  • Anshu Kumar,
  • Pradip Kumar Mondal,
  • Deepak Chopra,
  • Rupam Roy,
  • Sana Jindani,
  • Bishwajit Ganguly,
  • Chaithra Mayya,
  • Dhiraj Bhatia,
  • Vinod K. Jain

DOI
https://doi.org/10.1038/s41598-022-21407-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 16

Abstract

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Abstract Globally 3 billion people are consuming water with moderately high concentrations of fluoride and arsenic. The development of a simple point of care (PoC) device or home device for the detection of fluoride/arsenic ensures safety before consuming water. Till date, lateral flow assay (LFA) based PoC devices can detect nucleic acids, viruses and diseases. An aluminium complex of rhodamine B functionalized oxacalix[4]arene (L) was designed to execute the LFA-based PoC device. Initially, Al3+ and Fe3+ ions were involved in complexation with the rhodamine B functionalized oxacalix[4]arene (L), resulting C 1 (L-Al3+) and C 2 (L-Fe3+) complexes respectively. The receptor L, as well as the probes (C 1 , C 2 ), were characterized thoroughly using mass spectroscopy, FTIR, NMR, and EA. C 1 and C 2 were further utilized as recyclable probes for the detection of aqueous fluoride (21 ppb) and arsenate (1.92 ppb) respectively. The computational calculation indicates that upon complexation, the spirolactam ring opening at the rhodamine B site leads to optoelectronic changes. The consistency of LFA-based portable sensing device has been tested with water samples, synthetic fluoride standards and dental care products like toothpaste and mouthwash with concentrations ≥ 3 ppm. Moreover, fixed cell imaging experiments were performed to ascertain the in-vitro sensing phenomena.