eLife (Mar 2023)

Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens

  • Valentin Quiniou,
  • Pierre Barennes,
  • Vanessa Mhanna,
  • Paul Stys,
  • Helene Vantomme,
  • Zhicheng Zhou,
  • Federica Martina,
  • Nicolas Coatnoan,
  • Michele Barbie,
  • Hang-Phuong Pham,
  • Béatrice Clémenceau,
  • Henri Vie,
  • Mikhail Shugay,
  • Adrien Six,
  • Barbara Brandao,
  • Roberto Mallone,
  • Encarnita Mariotti-Ferrandiz,
  • David Klatzmann

DOI
https://doi.org/10.7554/eLife.81274
Journal volume & issue
Vol. 12

Abstract

Read online

T-cell receptors (TCRs) are formed by stochastic gene rearrangements, theoretically generating >1019 sequences. They are selected during thymopoiesis, which releases a repertoire of about 108 unique TCRs per individual. How evolution shaped a process that produces TCRs that can effectively handle a countless and evolving set of infectious agents is a central question of immunology. The paradigm is that a diverse enough repertoire of TCRs should always provide a proper, though rare, specificity for any given need. Expansion of such rare T cells would provide enough fighters for an effective immune response and enough antigen-experienced cells for memory. We show here that human thymopoiesis releases a large population of clustered CD8+ T cells harboring α/β paired TCRs that (i) have high generation probabilities and (ii) a preferential usage of some V and J genes, (iii) which CDR3 are shared between individuals, and (iv) can each bind and be activated by multiple unrelated viral peptides, notably from EBV, CMV, and influenza. These polyspecific T cells may represent a first line of defense that is mobilized in response to infections before a more specific response subsequently ensures viral elimination. Our results support an evolutionary selection of polyspecific α/β TCRs for broad antiviral responses and heterologous immunity.

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